TB-500
Thymosin Beta-4 Synthetic Acetylated Fragment Ac-LKKTETQ
TB-500 is a synthetic peptide containing the primary active actin-sequestering domain of naturally occurring Thymosin Beta-4. Preclinical investigations focus on its ability to regulate G-actin polymerization, promote cellular migration, and attenuate cardiac and myofibrillar fibrotic scarring.
Indexed for laboratory in vitro receptor binding assays and cellular synthesis research. Commercial materials are procured as non-clinical reagents under verified laboratory standards.
Chemical Structure & Metrics
Structured Chemical & Preclinical Profile
1. Chemical Identity & Sequence Architecture
TB-500 (Thymosin Beta-4 Synthetic Acetylated Fragment Ac-LKKTETQ) is an analytically characterized peptide with empirical formula C212H350N56O78S (MW 4963.50 g/mol). CAS Registry: 77591-33-4.
2. Primary Preclinical Signaling Axis
Investigated in preclinical assays for cellular signaling: G-actin sequestering via steric binding at the actin monomer cleft, regulating intracellular filament dynamics.
3. Physical Formats & Delivery Matrices
Synthesized predominantly as high-purity lyophilized powder cakes, with secondary pre-metered pen cartridges and solution atomizers cataloged across vendors.
4. Reconstitution & Analytical Handling
Recommended reconstitution with Bacteriostatic Water with 0.9% Benzyl Alcohol USP. Lyophilized cake stored at -20°C desiccated. Solution stable at 2°C to 8°C refrigerated (stable 21 days).
Signaling Pathways & Laboratory Handling Protocols
Documented Receptor Pathways & In Vitro Signaling
G-actin sequestering via steric binding at the actin monomer cleft, regulating intracellular filament dynamics.
Upregulation of matrix metalloproteinases to mobilize cellular migration across extracellular matrix lattices.
Stimulation of epicardial progenitor cell mobilization and coronary vessel morphogenesis in vitro.
Suppression of nuclear factor-kappa B (NF-kB) phosphorylation to blunt chronic inflammatory cascades.
Characterized by high systemic diffusivity due to low molecular weight relative to parent Thymosin Beta-4. Evaluated in cell migration scratch assays and skeletal muscle cellular motility models.
Handling Parameters
Peptide Dilution & Reconstitution Calculator
Compute stock concentrations, micro-pipetting draw volumes, and U-100 syringe units with zero procedural guesswork.
Slowly introduce bacteriostatic diluent against the internal glass vial wall. Swirl gently in a circular horizontal rotation. Never shake or vortex vigorously to prevent mechanical shearing of delicate peptide bonds. Store reconstituted stock at 2°C–8°C.
Partner & Vendor Catalogues for TB-500
Independent partner directory providing research-grade lyophilized vials, cartridges, atomizers, and reconstitution solvents.
Category hub featuring research-grade lyophilized vials, pens, and blends.
Full catalogue category hub with multiple mg vial sizes and blends.
Dedicated category hub listing research vials and cartridges.
UK-stocked research reagent catalogue hub featuring batch-verified peptide lots.
Global catalogue hub including international dispatch batches.
Academic Bibliography & Preclinical Studies for TB-500
Primary academic indexing sourced from PubMed and peer-reviewed biotechnology journals documenting molecular mechanisms and in vitro cellular response.
Thymosin beta 4 promotes dermal healing and regulates actin sequestration
Thymosin beta 4 induces adult epicardial neovascularization
Thymosin beta-4 protects against cellular apoptosis in ischemic cardiomyocytes
Frequently Asked Chemical & Analytical Questions
Authoritative scientific clarifications regarding TB-500 classification, molecular pathways, and laboratory protocols.
TB-500 (Thymosin Beta-4 Synthetic Acetylated Fragment Ac-LKKTETQ) is an analytically characterized peptide compound with empirical molecular formula C212H350N56O78S and molecular weight 4963.50 g/mol. It is indexed under CAS Registry Number 77591-33-4 and categorized in research literature under Tissue Repair & Angiogenesis.